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KMID : 0352519840210030001
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Korea Univercity Medical Journal
1984 Volume.21 No. 3 p.1 ~ p.9
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Experimental studies on the effect of albendazole against Clonorchis sinensis
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裵平元/Bae, Pyung-Weon
金洙鎭/林漢鍾/Kim, Soo-Jin/Rim, Han-Jong
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Abstract
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Newly developed albendazole (Zentel) is a broad spectrum anthelmintic with high activity against intestinal nematodes as well as trematodes and cestodes infections.
The present study was undertaken to evaluate in vitro and in vivo activities of albendazole. against adult worms of Clonorchis sinensis.
The morphological changes of C. sinensis obtained from experimentally infected rats treated with various dosages of albendazole were studied by means of scanning and transmission electron microscope.
The results are as follows:
1. The adult worms of C. sinensis were incubated in the medium containing albendazole with various concentrations. By 24 hours after incubation, none of the worms were dead in concentrations of 0.01 pg/ml to 10 pg/ml of albendazole.
2. In order to evaluate the effect of albendazole in vivo, experimentally infected rats with 50 metacercariae of C. sinensis each were treated orally with various dosages of albendazole.. In the result, anthelmintic effect was confirmed at the dosage of albendazole with 2 x 25mg/ kg x 2 days (total amounts of 100 mg/kg).
3. Macroscopical examination was made to the Clonorchis worms which were collected from the bile ducts of the rats autopsied at 24 hours after treatment with albendazole. The lack of intestinal contents was observed in most of worms from the groups treated with 1 x 200 mg/ kg and 2 x 25 mg/kg x 2 or 4 days, and the elongation of the anterior portion of the worm body was also observed in the worms from the groups treated with 2 x 25 mg/kg x 2 or 4 days.
4. In the scanning electron microscopic observation, many blebs were formed on the surface of the damaged tegument of the worms at 24 hours after treatment with albendazole (1 x 200 mg/kg and 2 x 25 mg/kg x 2 or 4 days).
5. The transmission electron microscopic observation indicated that degeneration of mito-
chondriae and destruction of tegumental syncytium of the worms at 24 hours after treatment with albendazole (1 x 200 mg/kg and 2X25 mg/kg x 2 or 4 days). In addition to the findings the degeneration of the intestinal lamellae in the caeca was also observed in the worms from the groups treated with same dosages of albendazole.
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